目的: 探讨LPA－COX－2 在小鼠肺纤维化发生发展过程中表达及作用。方法: 80 只小鼠随机分为博
来霉素组(BLM 组)及生理盐水组(NS 组)，采用ELISA 检测血清中溶血磷脂酸(LPA) 及环氧合酶－2(COX－2)
的表达;免疫组化法检测肺组织TGF－β1、EGFＲ、PGE2 及COX－2 的表达。结果: TGF－β1、EGFＲ、PGE2 及COX－
2 在NS 组肺脏中均有表达，但表达较弱;在BLM 组的表达强于NS 组。NS 组血清中LPA 少量表达，BLM 组血清
中LPA 呈高表达，有显著性差异(P＜0．01)。NS 组血清中COX－2 少量表达，BLM 组小鼠14d 时血清中COX－2
表达量明显增加(P＜0．01)，随着肺纤维化的加重，小鼠血清中的COX－2 表达水平逐渐降低，但较正常对照组仍
有明显表达(P＜0．01)。结论: LPA、COX－2、PGE2、TGF－β1 和EGFＲ 均在BLM 组高表达;LPA 可能通过TGF－β1
途径及EGFＲ 途径共同调节COX－2 及PGE2 表达。

Objective:To study the expression of LPA and COX－2 in the occurrence and development
of pulmonary fibrosis in rats． Methods: 80 rats were randomly divided into bleomycin group
(BLM group) and saline group (NS group)，using the ELISA tests as the expression of LPA and COX
－2 in serum; immuno－histochemical detection of lung tissue TGF－ beta 1，EGFＲ，of PGE2 and COX
－2． Ｒesults:TGF－ β 1，EGFＲ，PGE2 and COX－2 in the lungs of NS group were expressed，but the
expression is weak; the expression of BLM group was better than the NS group．The LPA in serum of
NS group was weakly expressed，while it was high expressed in BLM group，there was a significant
difference (P＜0．01)． COX－2 in the serum of NS group was weakly expressed，while the expression of
COX－2 in serum of BLM group was significant(P＜0．01) when mice on 14th day of the experiment．
With the aggravation of pulmonary fibrosis，the expression level of serum COX－2 decreased gradually，
but compared with the normal control group，the expression was still a significant difference (P＜ 0．
01)．Conclusion: LPA，COX－ 2，PGE2，TGF －β 1 and EGFＲ were highly expressed in the BLM
group;LPA may regulate the expression of COX－2 and PGE2 by TGF－β 1 pathway and EGFＲ path-
way． In the early stage，the expression of COX－2－PGE2 may be up－regulated mainly by LPA－EGFＲ，
and then participate in the inflammatory response of cells． In the later stage，the expression of COX－2
－PGE2 may be down regulated by LPA－TGF－ beta 1，and then participate in the formation of pulmonary
fibrosis．

Ｒ563．1

 收稿日期: 2018－05－20;修回日期: 2018－08－10
基金项目: 内蒙古医科大学科技百万项目(YKD2015KJBW018);内蒙古医科大学附属医院重大项目(NYFYZD2014001)
作者简介: 顾岩(1980－)，女，内蒙古医科大学附属医院呼吸与危重症医学科副主任医师，医学博士。
通讯作者: 付秀华，主任医师，E－mail:fuxiuhua555@ sohu．com 内蒙古医科大学附属医院呼吸与危重症医学科，010050