目的:研究新生儿缺氧缺血性脑病( HIE) 患儿IL-1茁、IL-2、IL-10、sIL-2R 和肿瘤坏死因子琢 (TNF-琢)水平及外周血T 细胞亚群的变化,探讨免疫学变化在HIE 发病中的意义,为其免疫治疗提供可能的理 论依据。方法:应用ELISA 法检测HIE 患儿及足月正常新生儿血清IL-1茁、IL-10、TNF-琢、IL-2、sIL-2R 水平, Ficoll 常规分离外周血单个核细胞,用免疫组化法检测HIE 患儿及足月正常新生儿CD+ 3、CD+ 4、CD+ 8、CD+ 4/ CD+ 8 细胞百分率。结果:HIE 患儿血清IL-1茁、IL-2、IL-10、TNF-琢、sIL-2R 水平较对照组增高( P<0. 05 或 P<0. 01);而HIE 患儿外周血CD+ 3、CD+ 4、CD+ 8 细胞数量降低(P<0. 01)。结论:HIE 患儿存在一定程度的免疫 功能紊乱。IL-1茁、IL-10、TNF-琢、IL-2、sIL-2R 和T 细胞亚群参与了HIE 的病理生理过程。

Objective:By measuring serum levels of IL-1,IL-10,TNF-琢,IL-2,sIL-2R and T cell subsets in neonates with hypoxic ischemic encephalopathy(HIE),and assessing their roles in path鄄 ogenesis of HIE and providing experimental evidence for HIE immune therapy. Methods: Blood samples were collected from 30 HIE and 30 normal birth neonates(control)in our hospital from 2004 to 2009. Serum IL-1茁,IL-10,TNF-琢,sIL-2R were measured by ELISA,peripheral blood mononuclear cells isolated by routine Ficoll-Hy paque,and the levels of CD+ 3,CD+ 4,CD+ 8 were detected in both groups. Results:The levels of IL-1茁,IL-10,TNF-琢,sIL-2R were higher in HIE group(P<0. 05); while IL-2 was lower(P<0. 01);CD+ 3,CD+ 4,CD+ 8 were decreased in HIE group(P<0. 05). Conclusion:The measured cytokines IL-1茁、IL-10、TNF-琢、IL-2、sIL-2R and T cell subsets were not normal in HIE new borns,which may play some roles in HIE pathogenesis.

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