目的:探讨上皮间质转化(EMT)在非小细胞肺癌(NSCLC) 侵袭转移中的作用,为NSCLC 的治疗和 预后预测提供新的实验依据。方法:利用免疫组化检测97 例EMT 相关标志物上皮性钙粘蛋白(E-cad)、茁-连 环素(茁-cat)和神经性钙粘蛋白(N-cad)、纤连蛋白(Fibronectin FN)、波形蛋白(Vimentin)在NSCLC 中的表达情 况。分析以上表达情况与肺癌临床病理特征的关系及其相关性。结果:(1)在97 例NSCLC 中,E-cad 、茁-cat 、 N-cad、FN、Vimentin 阳性表达率分别是50. 5% (49/97)、28. 9% (28/97)、8. 2% (8/97)、67. 0% (65/97)、34. 0% (33/97)。(2)E-cad 在不同组织学分级及淋巴结有无转移分组中其表达有显著差异(P<0. 05),茁-cat 在不同组 织分级阳性表达有显著差异(P<0. 05)。Vimentin 在淋巴结有无转移分组中表达有显著差异(P<0. 05),FN 在在 不同肿瘤大小、组织学分级及淋巴结有无转移分组中阳性表达有显著差异(P<0. 05)。(3) NSCLC 中,E-cad 与 茁-cat 表达存在正相关(P<0. 05)。(4)VIM 在NSCLC 中与E-cad 及茁-cat 表达呈明显负相关(P<0. 05),FN 表 达与E-cad 呈负相关( P<0. 05),而与茁-cat 表达无差异( P>0. 05)。(5) N-cad 在NSCLC 中阳性表达率为 8. 2%(8/97),在组织学类型及淋巴结有无转移分组中阳性表达有显著差异(P<0. 05)。结论:E-cad、茁-cat 在 NSCLC 中表达发生了下调,而N-cad、FN、VIM 表达升高,说明NSCLC 中存在EMT 现象。且与肿瘤侵袭转移相 关,均可以作为NSCLC 恶性程度、转移复发、预后的判断指标。

Objective:Investigate the relationship with clinicopathological features of the RelatedFN、Vimentin protein in NSCLC were 50. 5%(49/97)、28. 9% (28/97)、8. 2% (8/97)、34. 0% (33/ 97)and 67. 0%(65/97)respectively. (2)There was significant difference of expression rates of E-cad in the histological differentiation and lymph node metastases ( P < 0. 05). 茁- cat was significant difference of expression rates in the histological differentiation(P <0. 05). Vimentin was significant difference of expression rates in the lymph node metastases(P<0. 05). FN was significant difference of expression rates in the tumor size、the histological differentiation and lymph node metastases( P < 0. 05). (3)In NSCLC,There was a positive correlation between the expression of E-cad and 茁-cat. (4)In NSCLC,VIM was a negative correlation with the expression of E-cad and 茁-cat. 。There was a nagative correlation between the expression of FN and E-cad. (5)N-cadherin positive expression rate in NSCLC was 8. 2%(8/97),the grouping of the histological type and lymph node metastasis-positive expression were significantly different(P<0. 05). Conclusion:IN SNSCLC E-cad、茁-cat genes were down-regulated,especially 茁-catenin expression rate decreased significantly;N-cadherin,FN,VIM ex鄄 pression was elevated, indicating there exists the EMT phenomenon in NSCLC,indicating there exists the EMT phenomenon in NSCLC,and was related to invasion and metastasis. protein of EMT, analyze their role in NSCLC development and evaluate the correlation of their expression,and finally provide theoretical basis for clinical therapy of NSCLC. Methods:To detect the expression of the Related protein of EMT,E-cad、茁-cat and N-cad、FN、Vimentin in NSCLC through immunohistochemical detection,analysis of the above expression with lung cancer clinical pathological features and their relationship. Results:(1)The positive expression rates of E-cad、茁-cat and N-cad、

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