目的:体外原代培养人脑多形性胶质母细胞瘤细胞,应用不同细胞数量接种裸鼠脑内建立颅内胶质 瘤实验动物模型,观察其生长特性。 方法: 采用酶消化法原代培养 4 例人脑多形性胶质细胞瘤,分别取生长状 态良好的胶质瘤细胞悬液20滋L,按1. 0伊105 个/ 10滋L,1. 0伊106 个/ 10滋L,1. 0伊107 个/ 10滋L 立体定向接种于裸鼠 脑右侧尾状核区。 在整体、组织和细胞水平观察并记录肿瘤生长情况,于成瘤后第5wk 处死荷瘤鼠检测是否存 在肿瘤远处转移,同时对肿瘤组织行 HE 和免疫组织化学染色检测胶质瘤的病理学特征。 结果: 细胞悬液接种 可成功获得多形性胶质细胞瘤模型,成功率较高,但潜伏期延长,各种接种量的实验组成瘤率均为 100%,未见 颅外转移病灶,在组织病理学上接近人脑胶质瘤。 结论: 多形性胶质细胞瘤细胞接种裸鼠建立脑胶质瘤动物模 型,成瘤率高,颅内生长稳定,肿瘤组织病理学及形态学特性与人脑胶质瘤相似,可作为临床胶质瘤基础研究的 理想模型。

To culture the primary human glioblastoma cells in vitro and to establish a brain glioma model in nude mice with different cell number in order to study the growth feature of glioma. Methods:The glioma cells from 4 glioma patients were cultured primarily by the enzymatic digestion culture method. 20滋L of Well cultured glioma cells suspension containing 1. 0 伊105 / 10滋L, 1. 0伊106 / 10滋L,1. 0伊107 / 10滋L / 10滋L was stereotaxically inoculated into the right caudate nucleus of the rat brain. And then observed and recorded the growth of tumors in overall, tissue and cell Horizontal. Executed the nude mice at the fifth week after tumorn established,and resected their five main organs and the brain tissues. Performed HE stain and immuno - histochemistry examination to detect pathological characteristics of glioma. Results:Multiforme glioblastoma subcutaneous model closeto human glioma histopathology was successfully established by cell suspension inoculation,with high success rate and long latent period,but without other organs metastasis. Conclusion:The rat multiforme glioblastoma brain tumor model was successfully established with highly intracranial tumor formation rate, intracranial growth stable and no extracranial growth extension. This model resembles histopathological and morphological features of human glioma,which can be used as a perfect model to study glioma.

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