[关键词]
[摘要]
目的: 探讨低温下鄂尔多斯高原碱湖钝顶螺旋藻( S1 ) 质膜脂肪酸的变化及其与抗低温的关系，并与
非洲Chad 湖钝顶螺旋藻( S2 ) 进行比较。方法: 采用两相法提纯质膜，气相色谱法测定质膜类脂脂肪酸相对百分
含量。结果: 随着温度的降低，S1 和S2 质膜的总饱和脂肪酸均呈降低的趋势，总不饱和脂肪酸均呈增加的趋势;
软脂油酸( 16: 1) 和亚麻酸( 18: 3) 的含量在S1 中明显增加，在S2 中明显减少;而油酸( 18: 1) 和亚油酸( 18: 2) 的
含量正好相反，在S1 中明显减少，S2 中明显增加。结论: 不饱和脂肪酸在低温逆境中存在调节作用。推测S1 质
膜中与抗低温有关的关键脂肪酸可能是软脂油酸和亚麻酸; S2 中可能是油酸和亚油酸。S1 的抗寒性比S2
的强。
[Key word]
[Abstract]
Objective: To explore the variety of PM fatty acids of the Spirulina platensis( S1) from
alkaline lake in Erdos Plateau and its relationship with low temperature resistance under low temperatures
stress，and comparing them with that of S． platensis( S2) from Chad Lake in Africa at the sametime．
Methods: Using two － phase method to purify plasma membrane ( PM) ，gas chromatography
method for the determination of fatty acids relative contents of plasma membrane lipid． Results: As temperature
is lowered， the total saturated fatty acids in PM of both S1 and S2 show a decreasing trend;
while the total unsaturated fatty acids show an increasing tendency． Pamitoleic acid( 16 ∶1) and linolenic
acid( 18 ∶3) in S1 increase obviously，while in S2 both reduce significantly; oleic acid( 18 ∶1) and linoleic
acid ( 18 ∶2) contents are on the contrary，decreasing in S1 and increasing in S2 ． Conclusion: Unsatu-
rated fatty acids have the function of regulation under low temperature stress． It is speculated that key
fatty acids in S1 PM，which are involved in low temperature resistance，may be pamitoleic acid and linolenic
acid，especially the linolenic acid; while those in S2 may be of oleic acid and linoleic acid． S1 is
stronger than S2 on anti－low temperature stress．
[中图分类号]
R917
[基金项目]
收稿日期: 2013－01－09; 修回日期: 2013－05－03
基金项目: 国家自然科学基金项目( No． 30460104) ; 内蒙古自然科学基金项目( No． 200308020310) ;
内蒙古高等学校科学研究项目( NJ04036)
作者简介: 张三润( 1967－) ，男，内蒙古医科大学基础医学院讲师，医学硕士。
通讯作者: 杨茜，教授，E－mail: runsanzhang@ yahoo． com． cn 内蒙古医科大学， 010059
