[关键词]
[摘要]
目的: 提取并测定两不同产地蒙药云香十五味丸中黄酮类化合物的含量。方法: 以甲醇作为提取溶
剂，采用索氏提取器提取样品中的黄酮类化合物，以芦丁为对照品，采用分光光度法测定其含量。结果: 回归方
程为A = 0． 34452 C +0． 0222，r = 0． 9983，定量测定线性范围为16． 08 ～ 96． 48μg /mL，加样回收率为97． 33%
～ 100． 05%，RSD= 1． 14%。测得两不同产地蒙药云香十五味丸中的黄酮类化合物的平均含量分别为11． 13 mg /
g 与18． 52 mg /g。结论: 本法操作简便，重复性好，便于推广使用，适合于丸制剂药物中黄酮类化合物含量的
测定。
[Key word]
[Abstract]
Objective: To extract and determine the content of flavonoids in two kinds of Mongolian
medicines pills of fifteen ingredients of Yunxiang from different producing areas． Methods: Taking
methanol as solvent extraction and rutin as a reference substanc， to extract flavonoids from sample with
Soxhlet extraction device and to determine its content by using spectrophotometry． Results: The regression
equation is A= 0． 34452 C+0． 0222，r = 0． 9983， the linear range of quantitative determination was
16． 08 ～ 96． 48 g /mL ，and the recovery rate was 97． 33% ～ 100． 05，RSD= 1． 14%． The average determination
of flavonoids in two kinds of Mongolian medicines pills of fifteen ingredients of Yunxiang
were 11． 13 mg /g and 18． 52 mg /g respectively． Conclusion: The method is simple， repeatable，promotionable
and suitable to determine the content of total flavonoids in pills medicines．
[中图分类号]
R291． 2
[基金项目]
收稿日期: 2012－12－30; 修回日期: 2013－04－14
基金项目: 内蒙古自治区科技计划( 社发) 项目( 2012)
作者简介: 云学英( 1958－) ，女，蒙古族，内蒙古医科大学药学院教授，硕士研究生导师。
通讯作者: 马瑞莲，主任药师，E－mail:
ML3033@163． com 内蒙古医科大学附属医院药剂部， 010050
