目的:探讨PPARγ2 基因P12A 单核苷酸多态性与2 型糖尿病肾病的关系。方法: 所选对象根据
WHO 对糖尿病的标准，对照组55 例，2 型糖尿病组97 例。2 型糖尿病组根据每分钟尿蛋白排泄率早期肾病组
59 例和临床肾病组31 例。TaqMan 探针检测PPARγ2 基因Pro12Ala 基因型和等位基因。结果: PPARγ2 基因的
A 等位基因频率在正常组、早期肾病组和临床肾病组中分别为7． 7%、6． 8%和8． 1%，P12A 基因型及等位基因
的分布频率在正常组、早期肾病组和临床肾病组之间无显著性差异( P＞0． 05) 。调整病程后，应用logistic 回归分
析PPARγ2 基因Pro12Ala 单核苷酸多态性与2 型糖尿病肾病相关性，结果显示OR 值在肾病组的两组间比较
OR= 0． 966，95%IC 为0． 279 ～ 3． 344。在早期肾病组和临床肾病组比较，A 等位基因携带者在空腹胰岛素、胰岛
素抵抗指数、胰岛素敏感指数和尿蛋白排泄率与P 等位基因携带者比较有统计学意义( P＞0． 05) 。结论:
PPARγ2 基因Pro12Ala 单核苷酸多态性与2 型糖尿病肾病无明显的相关性。PPARγ2 基因A 等位基因是2 型糖
尿病肾病发生发展的保护基因。

Objective: To study relationship betwecn P12A single nucleotide polymorphism in peroxisome
proliferator－activated receptors gamma 2( PPAR gamma 2) and type 2 diabetic nephropathy．
Methods: According to WHO criteria for diagnosis of diabetes mellitus， the control group ( n = 55) ．
Type 2 diabetes mellitus ( n = 97) ． According to urinary albumin excretion in type 2 diabetic nephropathy，
morning group ( n = 59) and clinical group( 31) were included in this study． These subjects were evaluated
for the Pro12Ala single nucleotide poly－morphism in the PPAR gamma 2 using Taqman probe
in real－time Q－PCR． Results: A allele frequencies of Pro12Ala mutation of peroxisome proliferator－ac-
tivated receptors gamma 2( PPAR gamma 2) were 7． 7%、6． 8% 和8． 1% in control group and type 2
Diabetic nephropathy． There were no differences in genotype and allele frequencies between those three
groups( P ＞0． 05) 。After adjustment for course of disease were detected evidence for an interactin
effect between the Pro12Ala single nucleotide polymorphism and type 2 diabetes using logistic regression，
OR= 0． 966， 95% confidence interval 0． 279 ～ 3． 344． Between morning group and clinical group
A allele frequencies of Pro12Ala mutation of peroxisome proliferator － activated receptors gamma 2
( PPAR) were differences about insulin on an empty somach and insulin resistance idindex and insulin
sensitivity Index and urinary albumin excretion． Conclusion The Pro12Ala single nucleotide polymorphism
of peroxisome proliferator－activated receptors gamma 2 was not associated with diabetic nephropathy．
A allele of peroxisome proliferator－activated receptors gamma 2 could precent to progress in type
2 diabeteic nephropathy．
Key words: peroxisome

R589． 02

 收稿日期: 2013－04－17; 修回日期: 2013－06－19
基金项目: 内蒙古自治区自然科学基金( 20080404MS1126) ; 教育部春晖计划( 2006)
作者简介: 郝利霞( 1976－) ，女，内蒙古医科大学附属医院康复科主治医师，医学硕士。
通讯作者: 毕力夫，教授，博士生导师，E－mail: bilifu@ hotmail． com 内蒙古自治区卫生厅， 010010