目的: 探讨时间分辨荧光免疫分析( TRFIA) 检测乙肝病毒血清标志物的临床价值。方法: 用酶联
免疫吸附试验( ELISA) 和时间分辨荧光免疫分析对185 例血清标本进行乙肝标志物的定性、定量检测和比较，
对结果进行分析。结果: TRFIA 检测乙肝五项指标具有良好的准确性和重复性。与ELISA 相比，TRFIA 在
HBsAg、抗－HBs、HBeAg、抗－HBe的测定上差异无统计学意义( P＞0． 05) ，而在抗－HBc的测定上差异有统计学意
义( P＜0． 05) 。高、中浓度标本两种方法检测HBsAg 阳性均为10 例，而在低浓度中，TRFIA 阳性率要高于
ELISA。经两种方法检测，测得9 种模式，其中6 种模式两着检出的阳性例数接近，3 种模式( 4－5、5、全阴) 差异
较大。结论: TRFIA 法定量检测乙肝标志物的灵敏度更高，特异性更好，并有助于对治疗效果作出有效的判断。

Objective: To study the clinical application value of time－resolved fluorescence immunoassay(
TRFIA) for detection of serum markers of hepatitis B virus． Methods: Using enzyme immunoassay(
ELISA) and time－resolved fluorescence immunoassay respectively in 185 serum specimens，hepatitis
B markers were qualitatively and quantitatively detected and compared，analysis of the results． Results:
Five makers of hepatitis B was measured with good accuracy and repeatability by TRFIA． There
was no remarkable significance between ELISA and TRFIA in detecting HBsAg、anti－HBs、HBeAg ang
anti－HBe( P＞0． 05) ． But there was significant difference between ELISA and TRFIA in detecting anti－
HBc( P＜0． 05) ． In each 10 samples of high and medium concentrations of HBsAg，all were positive by
TRFIA and ELISA． However， the detection rate with TRFIA assay was higher than that with ELISA assay
in 10 samples of low concentration． There were nine modes in two methods，positive example was
dose to each other in six modes， there were difference in three modes． Conclusions: Time－resolved fluorescence
immunoassay for detection of serum markers of hepatitis B virus had good sensitivity and
good specificity and it is helpful to estimate the effect．

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