目的:为鄂尔多斯高原碱湖节旋藻的多样性研究提供基础资料和理论依据。方法: 本文对鄂尔多斯
高原碱湖钝顶节旋藻( Arthrospira platensis) 、乍得湖钝顶节旋藻( A． platensis) 及极大节旋藻( A． maxima) 的镍铁
氢化酶小亚基hoxY 基因进行了克隆与测序，并进行同源性及亲缘关系的分析。结果: 3 个节旋藻样品hoxY 基因
部分序列长均为479 bp。鄂尔多斯高原碱湖钝顶节旋藻hoxY 基因与不同来源的钝顶节旋藻的同源性高达
99． 5%，与极大节旋藻的同源性达99． 9%。A． maxima 与非洲乍得湖A． platensis 间的亲缘关系更近，而鄂尔多
斯高原碱湖钝顶节旋藻与乍得湖钝顶节旋藻的亲缘关系相对远。结论: hoxY 基因保守性高，属内不同样品间序
列变化很小。同种不同来源的钝顶节旋藻由于不同环境条件等因素的影响导致基因序列上碱基发生一定的
差异。

In this paper，the hoxY gene of hydrogenase in Arthrospira were cloned and sequenced，
which Arthrospira platensis from Alkaline Lakes in the Ordos Plateau，A． platensis from Lake Chad in
Africa and A． maxima from Lake Texcoco in Mexico． And their homology and genetic relationship were
also analysed，in the hope of providing elementary data and theoretical bases for the research of diversity
of Arthrospira from Alkaline Lakes in the Ordos Plateau． The results showed that the partial sequence
of hoxY gene in Arthrospira contained 479 bp． There was a high degree of sequence homology
between A． platensis from Ordos and A． platensis from Lake Chad，A． maxima which were 99． 5%
and 99． 9 % respectively． The genetic relationship between A． platensis and A． maxima were closer
than that from A． platensis from Ordos． Conclusion: The gene hox Y had a high conservation，in
which sequence of the different samples among the same genera had low variation． However，gene sequence
of the same species of Arthrospira which grew in different environments has variation．

Ｒ394． 3

 收稿日期: 2013 － 11 － 16; 修回日期: 2014 － 01 － 12
基金项目: 内蒙古自治区高等学校科学研究项目( NJzy08025)
作者简介: 王越( 1989 － ) ，女，内蒙古师范大学2011 级硕士研究生。
通讯作者: 恩和巴雅尔，副教授． E － mail: nmsdenhe@ imnu． edu． cn 内蒙古师范大学生命科学与技术学院， 010022