目的: 建立一种操作简便、成熟稳定的显微镜下大鼠70% 肝切除模型。方法: 显微镜下采用无血切
肝术制备大鼠70%肝切除( 肝左叶和中叶) 模型，术后观察大鼠一般情况，计算造模成功率和4wk 存活率，术后
24 h 超声检测肝后下腔静脉血流情况。结果: 手术时间为20． 5±3． 6 min，术中无明显副损伤，出血量少，术后苏
醒并存活超过24 h 标志造模成功，造模成功率为100%( 15 /15) ，造模成功大鼠术后一般状态良好，术后24 h 超
声检测肝后下腔静脉血流通畅，无明显狭窄。术后4 wk 存活率为100% ( 15 /15) 。结论: 显微镜下制备大鼠
70%肝切除模型操作简便、成功率高、可重复性强，为研究肝再生、急性肝功能衰竭、肝肿瘤和肝移植等提供了一
种理想的实验模型。

Objective: To establish a convenient，mature and stable 70%hepatectomy model in the
rat using an operating microscope． Method An experiment model of 70% hepatectomy in the rat were
prepared using an operating microscope， left and middle hepatic lobe were resected by bloodless hepatectomy
technique，postoperative general situation was observed， the operation success rate and survival
rate after 4 weeks were calculated， the blood stream of inferior vena cava under the remnant liver was
tested at 24 hours after operation by ultrasound． Results: The operation time was 20． 5±3． 6 min，no apparent
side－injury， less bleeding，postoperative recovery and survived for more than 24 hours deemed
successful，operation success rate was 100%( 15 /15) ， the rat survived after operation were in good condition，
the inferior vena cava under the remnant liver was patent without significant stenosis． The
survival rate after 4 weeks was 100% ( 15 /15) ． Conclusion: An 70% hepatectomy model in the rat
using an operating microscope has the advantages of convenient method，high successful rate and reproducibility，
which provides an ideal experimental model for the study of liver regeneration，acute liverfailure， liver cancer and liver transplantation．

R602

 收稿日期: 2014－04－19; 修回日期: 2014－06－08
基金项目: 国家自然科学基金( 81260073) ; 内蒙古自治区自然科学基金项目( 2010MS1126) ;
内蒙古高等学校研究项目( NJZY13421)
作者简介: 乔建梁( 1982－) ，男，内蒙古医科大学附属医院外科主治医师。
通讯作者: 孟兴凯，主任医师． E－mail: mxk6788@ gmail． com 内蒙古医科大学附属医院， 010050