[关键词]
[摘要]
目的: 采用超声法提取阿尔泰狗娃花中的多糖,并测定其含量。方法: 采用紫外-可见分光光度法,
以葡萄糖为对照品,苯酚-浓硫酸显色法测定阿尔泰狗娃花中多糖的含量。结果: 总多糖在2. 535-15. 21μg /mL
范围内呈良好线性关系,回归方程Y = 0. 0501X-0. 0015( r = 0. 9998,n = 6) ,阿尔泰狗娃花多糖含量为5. 645%,
RSD 为0. 65%。经方法学考察显示,稳定性、精密度、重现性均较好,平均回收率为96. 86%,RSD 为1. 45%。结
论: 紫外分光光度法测定阿尔泰狗娃花中的多糖,操作简便、灵敏度高、重现性好,稳定性高,可作为阿尔泰狗娃
花中多糖含量测定方法。
[Key word]
[Abstract]
Objective: To extract the polysaccharide of Heteropappus altaicus( Willd. ) novopokr by
ultrasonic extraction,and determine the polysaccharide content. Methods: Based on the standard
sample glucose, the polysaccharide content of Heteropappus altaicus( Willd. ) novopokr polysaccharide
was determined by phenol-sulfuric acid coloration and Ultraviolet and Visible( UV) spectrophotometry.
Results: The results showed that the absorbance has a linear relationship with the concentration in the
range of 2. 535 ~ 15. 21μg /mL,and the regression equation is Y= 0. 0501X-0. 0015( r = 0. 9998,n =
6) . The polysaccharide consent determined is 5. 645%with 0. 65%of RSD. The methodological evaluation
demonstrated that this method has good stability,precision and reproducibility. Also, the average
recovery rate is 96. 86% and RSD is 0. 65%. Conclusion: The UV - spectrophotometry can be
employed to determine the polysaccharide content of Heteropappus altaicus( Willd. ) novopokr,because
the method is convenient in operation and has high sensitivity,good repeatability and stability.
[中图分类号]
R917
[基金项目]
收稿日期: 2014-04-20; 修回日期: 2014-06-02
基金项目: 内蒙古医科大学大学生“英才培育”项目( 2013-2014)
作者简介: 杜艳青( 1980-) ,女,内蒙古医科大学药学院无机化学教研室讲师。
通讯作者: 王美玲,教授,E-mail: wangmeiling080@ sina. com 内蒙古医科大学药学院无机化学教研室, 010110