[关键词]
[摘要]
目的: 本实验拟通过测定GD 病人131 I 治疗前后外周血辅助性T 淋巴细胞膜表面CD30、CD195 的表
达，探讨GD 病人Th1 /Th2 平衡机制变化及其可能的临床意义。方法: 选择40 例GD 病人和40 例健康对照组为
研究对象，应用流式细胞术检测GD 病人131 I治疗前及治疗后6mo 的外周血淋巴细胞膜表面CD195、CD30 的变
化以及临床症状和甲状腺功能。结果: ( 1) GD 病人治疗前CD195 显著低于健康对照组( P＜0． 05) ; CD30 高于健
康对照组，CD195 /CD30 低于健康对照组( P＜0． 01) 。( 2) GD 病人131 I 治疗后6mo 34 例甲亢缓解。治疗后缓解
组CD195 上升，CD30 下降，与治疗前比较有统计学意义( P＜0． 05) ; CD195 /CD30 上升，较治疗前变化显著( P
＜0． 01) 。结论: ( 1) GD 的发病机理与病人外周血T 淋巴细胞膜中Th 1 /Th 2 平衡变化机制有关。( 2) GD 病
人131 I治疗缓解组Th 细胞膜表面CD195、CD30 及其比值的变化有助于更好的研究GD 的发生机制及对疾病短
期预后的判断。
[Key word]
[Abstract]
Objective: To examine the expression of CD195 and CD30 of the T－helper lymphocyte
( Th) from peripheral blood before and after 131 I therapy， in order to explore the balance mechanism of
Th1 /Th2 in Graves’disease ( GD) and the clinical significance． Methods: The expression level of
CD195 and CD30 of from 40 GD patients and 40 healthy controls were examined by Flow Cytometry．
The level of FT 3、FT 4 and TSH were also examined by chemiluminanscent． Results: ( 1) The expression
level of CD195 of GD patients without131 I therapy was lower than that of the healthy controls( P
＜0． 05) ． While CD30 level was higher than that of controls． The ratio of CD195 and CD30 was lower
than that of controls( P＜0． 01) ． ( 2) 34 GD patients with131 I treatment showed reduced hypothyroidism．
The CD195 level of GD patients showing reduced hyperthyroidism was increased and the CD30 was decreased(
P＜0． 05) ; the ration of CD195 /CD30 was increased and showed significant difference comparing
to the healthy controls( P＜0． 01) ． Conclusions: ( 1) The mechanism of GD has the correlation with
the balance of Th1 /Th2． ( 2) The level of CD195 and CD30 and the variation of ratio between them may
be involved in pathogenesis of GD， therefore it will be useful for studying the mechanism of GD and evaluation
of the short－term prognosis of 131 I therapy．
[中图分类号]
R817． 9
[基金项目]
收稿日期: 2014－04－21; 修回日期: 2014－06－20
基金项目: 内蒙古自治区自然科学基金项目( 2011MS1120)
作者简介: 席文琪( 1972－) ，女，内蒙古医科大学附属医院内分泌科副主任医师，医学硕士。
通讯作者: 皇甫建，主任医师，教授，硕士研究生导师，E－mail: hfj． 999@ 163． com 内蒙古医科大学附属医院内分泌科，
010050
