[关键词]
[摘要]
目的: 从蒙药诃子中提取多糖,并测定其含量。方法: 采用紫外分光光度法,以葡萄糖为对照品,用
苯酚-硫酸显色法测定诃子中多糖的含量。结果: 经方法学研究,多糖在3. 212-19. 27μg·mL-1 范围内呈线性
关系,回归方程Y= 0. 0432X +0. 0146( r = 0. 9996,n = 6) ,含量为15. 56%,平均回收率为100. 4%,RSD = 1. 60%。
结论: 采用紫外可见分光光度法测定蒙药诃子中多糖含量的方法简便、快速、灵敏,结果准确可靠,适用于诃子中
多糖含量的测定方法。
[Key word]
[Abstract]
Objective: To explore the extraction and content determination of polysaccharide in the
Mongolian medicine Terminalia chebula Retz. Methods: Phenol -sulfuric acid method and UV-PDA
spectrophotometer were used to determine content of polysaccharide in Terminalia chebula Retz. With
glucose as standard substance. Results: By the methodology study,polysaccharide had a linear relation
within the scope of 3. 212 to 19. 27μg /mL, the regression equation was Y = 0. 0432X +0. 0146 ( r
= 0. 9996,n = 6) , the content was 15. 56%, the average recovery was 100. 4%,RSD = 1. 60%. Conclusion:
UV-Vis spectrophotometry is simple, rapid, sensitive,accurate and reliable,which can be used
to determine polysaccharide in Mongolian medicine Terminalia chebula Retz.
[中图分类号]
R291. 2
[基金项目]
收稿日期: 2014-04-13; 修回日期: 2014-06-03
基金项目: 内蒙古医科大学大学生科技创新“英才培育”项目( 2014YCPY019)
作者简介: 于姝燕( 1983-) ,女,内蒙古医科大学药学院有机化学教研室讲师,理学硕士。
通讯作者: 罗素琴,教授,E-mail: luosuqing1954@ sina. com 内蒙古医科大学药学院, 010010