目的:观察卡维地洛( CAＲ) 对大鼠颈动脉损伤血管组织MMP－9、TIMP－1 表达的影响，探讨卡维地
洛防治再狭窄的机制。方法:雄性Wistar 大鼠90 只，随机分为假手术组、损伤组和CAＲ 组，后两组行颈动脉球
囊损伤术。三组均于术后1 d、3 d、7 d、14 d、28 d 处死大鼠。光镜下观察血管损伤后内膜增生情况，用免疫组化
和ＲT－PCＲ 法检测MMP－9、TIMP－1 在各组术后不同时间点的表达情况。结果: 与损伤组比较，术后14 dCAＲ
组内膜面积、内膜与中膜面积比值显著减少，管腔面积显著扩大( P＜0． 05) ; 与损伤组比较，CAＲ 组术后3 ～ 14
dMMP－9 表达显著减少( P＜0． 05) ，而TIMP－1 表达无显著变化。结论: 卡维地洛有效抑制大鼠颈动脉损伤后
MMP－9 表达，抑制了VSMCs 迁移、增殖，改善了细胞外基质的合成与降解平衡，从而减轻再狭窄。

Objective: To study the effects of carvedilol( CAＲ) on the the expression of MMP －9
and TIMP－1 in carotid artery－injured rats and explore the mechanisms of carvedilol in prevention of
restenosis． Methods: 90 Wistar rats were randomly divided into sham－operated group， injury group and
CAＲ group， and the model of carotid balloon injury were established in the latter two groups． The rats
were killed on the 1 st、3 rd、7 th、14 th、28 th day after injury． The intimal proliferation was observed by
optical microscope． The expression of MMP－9 and TIMP－1 at different time points were detected by the
method of immunohistochemistry and ＲT－PCＲ． Ｒesults: The intimal area( IA) and ratio of intimal and
medial area( IA/MA) in CAＲ group were significantly lower than those in injury group，while lumen area
was higer than its in injury group( P＜0． 05) ． Compared to injury group， the expression of MMP－9 significantly
decreased in CAＲ group at 3 rd to 14 th day after injury( P＜0． 05) ． While the expression of TIMP
－1 had no difference between two groups． Conclusion: CAＲ could reduce restenosis， the mechanism of
which may be inhibit the expression of MMP－9 in carotid artery－injured rats，VSMCs migration and proliferation
and improve balance of synthesis and degradation of extracellular matrix．

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 收稿日期:2014－09－10; 修回日期:2014－10－15
基金项目:内蒙古自治区自然科学基金项目( 2014MS08113)
作者简介:袁向珍( 1973－) ，女，蒙古族，内蒙古医科大学附属医院保健中心副主任医师，医学博士。