目的:建立四味光明盐汤散中胡椒碱的含量测定方法。方法: 高效液相色谱法。Phenomen C18 色谱
柱( 250 mm×4． 6 mm，5μm) ;甲醇－水( 77: 23) 为流动相; 流速为1． 0 mL/min; 检测波长为343nm; 柱温30℃。结
果:胡椒碱在50． 80 ng ～ 508． 0 ng 范围内与峰面积积分值呈良好的线性关系，回归方程为y = 664890 x+53019，r
= 1． 0000。低、中、高浓度平均加样回收率分别为99． 31%、102． 4%、102． 4%，ＲSD% 分别
为0． 84%、0． 74%、0． 45%。结论:该方法准确、精密、重复性好，可作为四味光明盐汤散中胡椒碱的含量测定
方法。

Objective: To establish the determination method of Piperine in Siwei Guangmingyan
Tangsan． Methods: Phenomen C18 column 250 mm×4． 6 mm，5μm) was used in HPLC assay; mobile
phase was methanol－water( 77: 23) ; detection wavelength was 343nm; flow rate was 1． 0 mL /min; column
temperature was 30℃． Ｒesults: The Calibration curve of Piperine was linear in the range of 50． 80
ng ～ 508． 0 ng the regresstion equation was y = 664890 x +53019，r = 1． 0000，The lower，middle and
high concentration of the average recovery were 99． 31%，102． 4%，and 102． 4%，ＲSD% were
0． 84%，074%，0 ． 45%． Conclusion: The method was accurate，precise and had good reproducibility，
which can be used as determination method of Piperine in Siwei Guangmingyan Tangsan．

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 收稿日期:2014－09－25; 修回日期:2014－11－26
基金项目:内蒙古自治区政府蒙药现代化重大项目( MX2005ZB006)
作者简介:史文燕( 1991－) ，女，蒙古族，内蒙古医科大学2012 级在读硕士研究生。
通讯作者:王玉华，教授，E－mail: yuhuawang59@163． com 内蒙古医科大学药学院， 010010