目的:建立测定血清中阿米替林( AM) 及其产物N－去甲阿米替林( N－AM) 浓度HPLC 法。方法: 血
清样品碱化后采用乙醚萃取，其有机相吹干检测。以甲醇∶ 乙腈∶ 水∶ 正丁胺∶ 冰乙酸( 55 ∶90 ∶45 ∶2 ∶1． 5) 为流
动相，检测波长为254nm，VP－ODS 柱。结果:血清中阿米替林( AM) 及其产物N－去甲阿米替林( N－AM) 线性范
围均为25 ～ 400μg /L，方法回收率分别为92． 3% ～ 125． 7% 及109． 9% ～ 121． 8%，日内ＲSD 分别为5． 52%
～ 7． 70%及1． 76% ～ 9． 46%，日间ＲSD 分别为4． 59% ～ 7． 70%及1． 96% ～ 9． 59%。结论:本方法在阿米替林血
药浓度监测应用上具有可操作性强，重现性好，临床监测周期短等优点，适合人体药动学研究。

Objective: To develop a method of HPLC for the simultaneous determination of Amitriptyline
and its active metabolite N－desmethyl Amitriptyline in serum． Methods: N－desmethyl Amitriptyline
and Amitriptyline in serum were extracted by ether and the oganic phase must blow dry to detected．
The chromatographic separation was performed on the VP －ODS column using the mixture of
methanol: water: butyl－Amine: glacial acetic acid( 55: 90 ∶2 ∶1． 5) as the mobile phase． The eluate was
monit－ored at 229nm． Ｒesults: The linearity was obtained over the range of 31． 25 ～ 500μg /Lwith the
recoveries 92． 3% ～ 125． 7% and 109． 9% ～ 121． 8% for N － desmethyl Amitriptyline and
Amitriptyline． respectively． The intraday ＲSD were 5． 52% ～ 7． 70% and 1． 76% ～ 9． 46%，and the
inter－day ＲSD were 4． 59% ～ 7． 70%and 1． 96% ～ 9． 59%， for N－desmethyl Amitriptyline and Amitriptyline．
respectively． Conclusion: The amitriptyline blood concentration has a good operability， reproducibility
and short clinical monitoring periodidty，Which suit for human pharmacokinetic．

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 收稿日期:2014－09－24; 修回日期:2014－11－23
基金项目:内蒙古自然科学基金项目( 2009MS1155)
作者简介:赵金栓( 1964－) ，男，内蒙古精神卫生中心精神科临床检验副主任技师。