目的: 探讨NDRG 2 基因在结直肠癌组织中mRNA 的表达情况及其与临床病理特征之间的关系。
方法: 通过实时定量PCR 检测68 例结直肠癌组织及其配对癌旁组织中NDRG 2 mRNA 的表达情况，同时完成5a
随访，进而分析病人临床病理特征与术后生存率之间的关系。结果: ( 1) NDRG 2 在68 例结直肠癌组织中的mＲNA
表达量明显低于其配对癌旁组织( P ＜ 0． 01) ，差异有统计学意义; ( 2) TNM 分期早( TNM 1) 的结肠癌组
NDRG 2 mRNA 的表达量高于分期晚( TNM 2 /3 /4) 的结肠癌组( T1 VS． T2 + 3 + 4，P = 0． 04) ; 而其余临床病理特
征对于结直肠癌组NDRG 2 mRNA 表达量的影响无显著差异性( P ＞ 0． 05) ; ( 3) NDRG 2 表达水平与结直肠癌病
人生存率未见明显相关性( P ＞ 0． 05) 。结论: NDRG 2 基因参与结直肠癌的发生发展过程，但具体机制尚未明
确。NDRG 2 在核酸层面的表达与部位、分化程度、浸润深度、Dukes 分期、淋巴结侵犯、术前干预、复发及转移均
无明显关系，仅与TNM 分期有关，这为进一步探讨结直肠癌的分子诊断及治疗提供理论依据。

Objective: To explore the mRNA expression of NDRG 2 which is considered as candidate
tumor － suppressor genes in colorectal carcinoma and the relationship with clinical pathological
features． Methods: NDRG 2 mRNA levels were examined by real － time quantitative PCR in a cohort of
68 human colorectal carcinomas and 68 paired normal tissues． The correlation between NDRG 2 mRNA
expression with clinicopathological features and survival followed up for 5 years after surgery was analyzed．
Results: ( 1) NDRG 2 mRNA levels were found lower in tumor samples than in the normal tissues
( P ＜ 0． 01) ; ( 2) The mRNA expression level of NDRG 2 was higher in patients with TNM 1 compared
with TNM 2，3and 4( T 2，3and 4 compared to TNM 1 P = 0． 04) ，but there was no significant differences
of NDRG 2 mRNA expression between different groups of location differentiation invation Dukes
－ staging lymph node metastasis preoperation intervention，recurrence and distant metastasis ( P ＞
0． 05) ; ( 3) The expression levels of NDRG 2 were no statistical significance with patients survival．
Conclusion: NDRG 2 plays an important part in the progress of colorectal tumor development， invasion
or metastasis with unclear mechanism． NDRG 2 transcript levels revealed significant differences between
varying TNM staging，which provides the guiding for the clinical diagnosis and prognosis in future．

R735． 3

 收稿日期: 2016 － 01 － 13; 修回日期: 2016 － 05 － 26
基金项目: 内蒙古自然科学基金( 2013MS1189)
作者简介: 卢佳佳( 1992 － ) ，女，内蒙古医科大学2014 级在读硕士研究生。
通讯作者: 刘彩霞，主任医师，硕士研究生导师，E － mail: lcxzhg1974@ 163． com 内蒙古医科大学附属医院肿瘤内
科， 010050