目的: 测定蒙药参竹精片中总多糖的含量。方法: 采用紫外分光光度法，以葡萄糖为对照品，苯酚－
硫酸为显色剂，检测波长为487nm，测定样品溶液吸光度，计算总多糖的含量。结果: 葡萄糖浓度在
20． 04 ～ 100． 21μg·mL ^－1范围内与吸光度呈良好的线性关系，回归方程为Y = 1． 1819 X － 0． 0507( r = 0． 9990，n
= 6) ，参竹精片中总多糖的平均含量为0． 5314%，平均加样回收率为99． 37%，ＲSD 为0． 92%。结论: 采用苯酚－
硫酸法测定蒙药参竹精片中总多糖含量的方法简单、准确、重现性好，适用于参竹精片中总多糖的含量测定。

Objective: To determine total polysaccharides content of Shenzhujing Tablets． Methods:
UV － vis spectrophotometry was used to determine content of total polysaccharide in Shenzhujing
Tablets． The experiment was carried out with the glucose as comparison，phenol － sulfuric acid as developer．
487nm as detection wavelength． The content of total polysaccharides was calculated by determining
the absorbancy of sample solution． Results: The glucose shows a good linear relationship with absorbancy
at range of 20． 04 ～ 100． 21μg． mL ^－1 ． The regression equation is Y = 1． 1819 X － 0． 0507( r =
0． 9990，n = 6) ． The content of total polysaccharides in Shenzhujing Tablets was 0． 5314%． The average
sample recovery rate was 99． 37%( RSD = 0． 92%，n = 6) ． Conclusion: Phenol － sulfuric acid method
was simple accurate， reproducible and suitable for content determination of total polysaccharides in
Shenzhujing Tablets．

R284． 1

 收稿日期: 2016 － 01 － 03; 修回日期: 2016 － 05 － 26
基金项目: 呼和浩特市科技局( 2011 重－ 1 － 5 － 1)
作者简介: 张爱武( 1973 － ) ，女，内蒙古医科大学附属医院药剂部主任药师。
通讯作者: 周秀芳，主任药师，E － mail: 1162250207@ qq． com 内蒙古医科大学附属医院， 010050