目的: 在单因素的基础上，以乙醇溶为溶剂，采用响应曲面法优化超声提取漏芦花中总黄酮的工艺
条件。方法: 在乙醇浓度、提取时间、提取温度和液料比等单因素实验的基础上，以总黄酮得率为响应值，采用响
应曲面法中的Box－Behnken 模式对提取工艺进行研究。结果: 最佳工艺参数为乙醇体积分数70%、提取温度
70℃、料液比24．98、提取时间180min．在此条件下漏芦花总黄酮含量为0．1625μg /g。结论: 该优化工艺稳定可行，
可利用响应模型对实验结果进行预测。

Objective: On the basis of single factor，with water and ethanol as solvent， the surface
method is adopted to optimize the extraction process conditions of total flavonoids in the Rhaponticum
uniflorum．Methods: On the basis of four single factors， such as ethanol concentration， ratio of solid to
liquid， temperature and time， the extraction technology of the total flavonoids is studied by the Box－Behnken
design of response surface methodology．Result: The optimized extraction conditions are as follows:
ethanol concentration is 70%，solid － liquid ratio is 24．983，extractiontemperature is 70℃，
extraction time is 180 min． The experimental result confirmed that under the optimal conditions， the
yield of total flavonoids was up to 0．1625μg /g．Conclusion: The extraction technology was feasible and
the response model was used to predict the experimental results．

R943

 收稿日期: 2016－03－03; 修回日期: 2016－08－09
基金项目: 内蒙古医科大学青年创新基金( qncx2012024)
作者简介: 刘丽霞( 1977－) ，女，内蒙古巴彦淖尔市医院药剂科主管药师。
通讯作者: 包小妹，副教授，E－mail: amei0478@ 126．com 内蒙古医科大学药学院， 010110