目的: 通过比较NaNO₂－Al( NO₃)₃－NaOH 法和AlCl₃法的差异，建立荞麦降糖颗粒中总黄酮含量的
比色分析方法。方法: 以乙醇为溶剂进行回流提取，并以芦丁为对照品，分别以NaNO₂ －Al( NO₃)₃ －NaOH 和
AlCl₃为显色剂，分别在516nm 和417nm 处用可见分光光度法测定荞麦降糖颗粒中总黄酮含量。结果: 建立硝酸
铝法芦丁浓度－吸光度关系的回归方程方程式y = 0．01201x－0．0291，R² = 0．9994，回收率为100．2%。同时也建立
三氯化铝法芦丁浓度－吸光度关系的回归方程方程y = 0．02992+0．00473，R² = 0．9996，回收率为99．75%。结论:
AlCl₃法操作简便快速、专属性强，更适用于荞麦降糖颗粒中总黄酮的含量测定。

Objective: By comparing the difference between the NaNO₂ － Al ( NO₃)₃ － NaOH
methods and AlCl₃methods， to set up a Colorimetric assay for the determination of total flavones in the
Buckwheat Hypoglycemic granule．Methods: Extract the total flavones by ethanol，with rutin as standard
sample，NaNO₂－Al( NO₃)₃－NaOH and AlCl₃ as color reagents， the flavonoids content was determined
by VlS－spectrophotometry at the wavelength of 516nm and 417nm．Result: The regression equation of
NaNO₂－Al( NO₃)₃－NaOH methods was y = 0．01201x－0．0291，R² = 0．9994， the average recovery of this
method was 100．2%． The regression equation of AlCl3 methods was y = 0．02992 x + 0．00473，R² =
0．9996， the average recovery of this method was 99．75%．Conclusion: The method of spectmphotometry
in the AlCl₃ is simple， rapid，highly accurate，and suitable for quality control of Buckwheat Hypoglycemic
granule．

R917

 收稿日期: 2016－11－02; 修回日期: 2016－02－11
基金项目: 内蒙古自治区医疗卫生科研计划项目( 201302049)
作者简介: 白玉琴( 1993－) ，女，内蒙古医科大学2014 级在读硕士研究生。
通讯作者: 王焕芸，教授，E－mail: whuanyun999@ 163．com 内蒙古医科大学， 010110