目的: 通过探讨两栖动物蟾蜍的胚胎提取物与恶性增殖细胞的相互作用，从中筛选能够使癌细胞走
向凋亡的因子。方法: 将发育到不同阶段的蟾蜍胚胎提取物诱导体外培养的肝癌细胞，利用倒置显微镜进行同
步活体检测、荧光染色及电子显微镜观察等不同手段检测。结果: 发育早期的胚胎诱导肝癌细胞表现出了明显
的凋亡特征，看到的染色质在核膜周边聚集、细胞质空泡化。结论: 初步推断这些胚胎提取物对肝癌细胞具有诱
导凋亡的作用。

Objective: Through the discussion of the apoptosis － induced effects of the embryo
extract and cancer cell， selection the factors it can make cancer cell to apoptosis．Methods: Using the
embryo extracts of different stages to induced liver cancer cells．By inverted microscope for simultaneous
live detection， fluorescence staining and electron microscopy．Results: The early of embryos induced the
hepatocellular showed apoptosis characteristics．Conclusion: It is concluded that the embryo extracts
can induce apoptosis in hepatoma cells．

R392．12