[关键词]
[摘要]
目的: 研究SH－SY5Y 细胞中谷氨酰胺对Hsp70 的表达的影响。方法: 培养SH－SY5Y 细胞并用谷氨
酰胺干预后，分别使用PCＲ 和Western blot 检测SH－SY5Y 细胞中Hsp70 的表达; 用HSF－1 siＲNA 转染SHSYSY
细胞，用谷氨酰胺干预后，用PCＲ 和Western blot 检测SH－SY5Y 细胞中HSF－1 及Hsp70 的mＲNA 表达水
平及蛋白表达水平。结果: 培养SH－SY5Y 细胞并用谷氨酰胺干预后，SH－SY5Y 细胞中Hsp70 mＲNA 表达水平
及蛋白水平及均上调; 用HSF－1 siＲNA 转染SH－SYSY 细胞，即使用谷氨酰胺干预，SH－SY5Y 细胞中HSF－1 及
Hsp70 的mＲNA 表达水平及蛋白表达水平均下调。结论: 谷氨酰胺可以上调SH－SY5Y 细胞中Hsp70 的表达，且
这种上调呈HSF－1 依赖性。
[Key word]
[Abstract]
Objective: To research the influence of glutamine to Heat shock protein 70 in SHSY5Y
neuroblastoma cell． Methods: In present study，we investigated the promotion of Hsp70
expression in SH － SY5Y neuroblastoma cells by glutamine，which has recently been recognized to
induce Hsp70 expression，and then we investigated the role of HSF－1 in the Hsp70 promotion by glutamine．
Ｒesults: Gln treatment f significantly upregulated the Hsp70 mＲNA level．Gln treatment also promoted
the Hsp70 protein level．The Hsp70 was also significantly downregulated in both protein and mＲNA
levels，compared to the siＲNA control － transfected cells． Conclusion: Glutamine upregulates the
Hsp70 expression in SH－SY5Y cells．glutamine，and that was HSF－1－dependent．
[中图分类号]
Ｒ392．11
[基金项目]
