摘要 目的 比较内蒙古地区蒙汉族非小细胞肺癌(Non-small cell lung cancer,NSCLC) 患者放疗前后淋巴细胞亚群计数及TNF-α、IL-1β、IL-6、IL-10含量的变化，并探讨放疗对内蒙古地区蒙汉NSCLC患者机体免疫功能的影响。方法 收集2015年10月至2017年10月就诊内蒙古医科大学附属医院行放疗的NSCLC患者共60例，其中蒙古族患者20例，汉族患者40例。采集放疗前后患者血清，利用流式细胞仪检测T淋巴细胞及其亚群、NK细胞计数，ELISA法检测TNF-α、IL-1β、IL-6、IL-10含量，统计分析放疗前后蒙汉族之间各免疫指标的差异以及同一民族内部各指标的改变与放疗的关系。结果 对蒙汉族NSCLC患者放疗前后机体免疫状态进行分析，发现放疗前蒙古族患者的NK细胞计数高于汉族患者的NK细胞计数，两者之间具有统计学差异（P=0.038），其余检测指标在蒙汉族之间差异均无统计学意义（P>0.05)。在放疗后分析发现，汉族患者 IL-1β水平高于蒙古族患者，差异具有统计学意义（P=0.007），但是其他免疫指标改变均无统计学差异（P>0.05)。同时，对蒙古族NSCLC患者放疗前后自身机体免疫指标的检测发现，与放疗前相比较，在放疗后患者的Ｔh细胞计数（P=0.013）和Th/Tc细胞比率（P=0.049）均降低，血清中IL-6（P=0.003）、IL-1β（P=0.001）、IL-10（P=0.001）的表达也降低。在汉族NSCLC患者放疗前后机体免疫状态分析后发现，其治疗前后总Th细胞（P=0.002）、IL-6（P=0.001）、IL-10（P=0.004）变化改变有统计学差异。 结论 本研究通过检测内蒙古地区蒙汉族NSCLC患者放疗前后淋巴细胞亚群计数及血清中TNF-α、IL-1β、IL-6、IL-10的表达，证实蒙汉族NSCLC患者免疫功能具有差异，同时放疗对蒙汉族NSCLC患者免疫功能产生不同的影响，但仍需扩大样本量进一步探讨放疗对两者之间不同免疫通路的影响机制。

Abstract Objective To compare the changes of lymphocyte subpopulation counts and the levels of TNF-α, IL-1β, IL-6, and IL-10 before and after radiotherapy in Mongolian and Han non-small cell lung cancer (NSCLC) patients in Inner Mongolia. And to investigate the effect of radiotherapy on the immune function of patients with NSCLC in Mongolia and Han in Inner Mongolia. Methods A total of 60 patients with NSCLC who underwent radiotherapy at the affiliated hospital of Inner Mongolia Medical University from October 2015 to October 2017 were collected, including 20 Mongolian patients and 40 Han patients. The serum of patients before and after radiotherapy was collected, and the T lymphocytes and its subpopulations, NK cell counts were detected by flow cytometry, and the contents of TNF-α, IL-1β, IL-6, and IL-10 were detected by ELISA. The differences between the various immune indicators and the relationship between changes in various indicators within the same ethnic group and radiotherapy. Results The immune status of Mongolian and Han NSCLC patients before and after radiotherapy was analyzed. It was found that the NK cell count of Mongolian patients before radiotherapy was higher than that of Han patients. There was a statistical difference between the two (P = 0.038). There was no significant difference between Mongolian and Han nationality (P> 0.05). Analysis after radiotherapy revealed that the IL-1β level in Han patients was higher than that in Mongolian patients, and the difference was statistically significant (P = 0.007), but there were no statistical differences in other immune indicators (P> 0.05). At the same time, the detection of autoimmune indicators of Mongolian NSCLC patients before and after radiotherapy showed that compared with before radiotherapy, the patients'' Th cell count (P = 0.013) and Th / Tc cell ratio (P = 0.049) were reduced after radiotherapy. The expressions of IL-6 (P = 0.003), IL-1β (P = 0.001), and IL-10 (P = 0.001) in serum were also decreased. Analysis of the immune status of Han NSCLC patients before and after radiotherapy revealed that there were statistically significant changes in changes in total Th cells (P = 0.002), IL-6 (P = 0.001), and IL-10 (P = 0.004) before and after treatment. Conclusion In this study, the lymphocyte subpopulation counts and serum TNF-α, IL-1β, IL-6, and IL-10 expressions in Mongolian and Han NSCLC patients before and after radiation therapy were detected in Inner Mongolia, and the immune function of Mongolian and Han NSCLC patients was different. Radiotherapy has different effects on the immune function of Mongolian and Han NSCLC patients, but the sample size needs to be expanded to further explore the mechanism of the influence of radiotherapy on different immune pathways between the two groups.

内蒙古科技计划项目