目的 观察绝经前子宫内膜癌患者组织内TESTIN、酶和张力蛋白同源物基因 (phosphatasetensinhmmlogydeletedten,PTEN)蛋白表达情况,分析TESTIN与PTEN蛋白间的相关性。方法 回顾性分析,选择医院2016年10月至2019年10月期间接受子宫内膜癌切除治疗并经病理组织活检证实为子宫内膜癌的患者100例作为病例组,另纳入同期在医院经分段诊刮术刮出子宫内膜组织蜡块标本,并证实为良性病变的患者33例作为良性对照组。检测并比较两组病理组织的TESTIN、PTEN蛋白表达情况,分析绝经前子宫内膜癌组织中TESTIN、PTEN蛋白表达的相关性,比较不同临床病理特征子宫内膜癌组织的TESTIN、PTEN蛋白阳性率。结果 TESTIN、PTEN蛋白在绝经前子宫内膜癌组织中阳性表达减弱,在子宫内膜良性病变组织中呈强阳性表达；较良性对照组,病例组子宫内膜组织中检出的TESTIN、PTEN蛋白阳性率低,组间比较差异有统计学意义(P＜0.05)；子宫内膜组织中TESTIN、PTEN蛋白间呈正相关(r=0.867,P＜0.05)；不同年龄段、病理分化子宫内膜癌组织TESTIN、PTEN蛋白阳性检出率比较差异无统计学意义(P＞0.05)；高临床分期、肌层浸润严重、有淋巴结转移者阳性检出率较其他患者更低,差异有统计学意义(P＜0.05)。结论 TESTIN、PTEN蛋白在绝经前子宫内膜癌中表达缺失,二者相互影响、相互作用,可能参与了疾病的进展、浸润与转移。

Objective To observe the expression of TESTIN and phosphatase and tensin hmmlogy deleted ten (PTEN) protein in tissues of premenopausal endometrial cancer, and to analyze the correlation between TESTIN and PTEN protein. Methods The retrospective analysis was performed. 100 patients who underwent resection of endometrial cancer from October 2016 and October 2019 and were confirmed as endometrial cancer by pathological biopsy were selected as the case group. 33 patients who were diagnosed as benign lesions by scraping the endometrial tissue paraffin-embeded samples from fractional curettage in the hospital during the same period were included in the benign control group. The expression of TESTIN and PTEN protein in pathological tissues of two groups were detected and compared. The correlation between TESTIN and PTEN in premenopausal endometrial cancer tissues was analyzed. The positive rate of TESTIN and PTEN protein in endometrial cancer tissues with different pathological features was compared. Results The positive expression of TESTIN and PTEN protein in premenopausal endometrial cancer tissues weakened, and they showed strongly positive expression in benign endometrial lesions tissues; Compared with the benign control group, the positive rate of TESTIN and PTEN protein in endometrial tissues of the case group was lower, and the difference between groups was statistically significant (P<0.05); There was a positive correlation between TESTIN and PTEN protein in endometrial tissues (r=0.867, P<0.05); There was no statistical difference in the positive detection rate of TESTIN and PTEN protein in endometrial cancer tissues with different ages and pathological differentiation (P>0.05); The positive detection rate in patients with high clinical staging, severe muscular infiltration, and lymph node metastasis was lower than that in other patients (P<0.05). Conclusion The expression of TESTIN and PTEN protein is lost in premenopausal endometrial cancer. The two interact with each other and may be involved in disease progression, invasion and metastasis.