摘要：目的：探究干眼症（DES）患者结膜上皮细胞及泪液中硬脂酰辅酶 A 去饱和酶-1（SCD-1）、溶菌酶 C（LYSC）的表达及意义。 方法：随机抽取在内蒙古医科大学附属医院干眼门诊就诊的 DES 患者 55例（DES 组）及非妊娠健康人群 50 例（对照组），利用实时荧光定量 PCR（qRT-PCR）技术检测两组研究对象结膜上皮细胞及泪液中 SCD-1 水平，利用微量快速比浊法测定 LYSC 含量，比较 DES 组及对照组结膜上皮细胞及泪液中 SCD-1、LYSC 水平，DES 组治疗前后 SCD-1、LYSC 水平及各项临床指标的变化，利用Pearson 分析 DES 患者结膜上皮组织及泪液中 SCD-1、LYSC 水平与各项临床指标变化的相关性。结果：DES 组患者结膜上皮细胞、泪液中 SCD-1、LYSC 水平与对照组相比均显著降低（P<0.05）；与治疗前相比，DES 组患者治疗后结膜上皮细胞及泪液中 SCD-1、LYSC 水平升高（P< 0.05），BUT 延长，SIT 值增加，CFS 评分降低（P<0.05）；Pearson 结果显示，结膜上皮组织及泪液中所含 SCD-1、LYSC 水平与BUT、SIT 结果呈正相关（P<0.05），与 CFS 评分呈负相关（P<0.05）。结论：DES 患者结膜上皮细胞和泪液中 SCD-1、LYSC 水平均低于健康对照组，治疗后 SCD-1、LYSC 水平均高于治疗前，提示 SCD-1、LYSC水平变化可能与泪液中的脂质存在某种关系，参与 DES 症状的发生、发展。

Abstract：Objective:To investigate the expression and significance of stearoyl-CoA desaturase-1 (SCD-1) and lysozyme C (LYSC)in conjunctival epithelial cells and tears of patients with dry eye disease (DES).Methods: 55 cases of DES patients (DES group) and 50 Non-pregnant healthy people (control group) treated in dry eye outpatient department of affiliated hospital of Inner Mongolia Medical University were selected to detect conjunctiva in two groups using real-time fluorescence quantitative PCR (qRT-PCR) technology. The levels of SCD-1 in epithelial cells and tears were determined by the rapid turbidimetry method. The levels of SCD-1 and LYSC in conjunctival epithelial cells and tears in the DES group and the control group were compared. The levels of SCD-1 and LYSC in the DES group before and after treatment;Pearson was used to analyzed the correlation between SCD-1 and LYSC levels in conjunctival epithelial tissue and tear fluid and changes in various clinical indicators in patients with DES. Results:The levels of SCD-1 and LYSC in conjunctival epithelial cells and tears were significantly lower in the DES group than in the control group (P<0.05);Levels of SCD-1 and LYSC in conjunctival epithelial cells and tears increased in the DES group as compared to pre-treatment (P<0.05), BUT extended, SIT value increases, the CFS score lower (P<0.05); Pearson, according to the results of conjunctival epithelial tissue and tears of SCD-1, LYSC level and regress, SIT positively correlated (P<0.05), negatively correlated with CFS score (P<0.05). Conclusions:Levels of SCD-1 and LYSC in conjunctival epithelial cells and tears of DES patients were lower than those of healthy control group, Both SCD-1 and LYSC levels were higher after treatment than before, which is suggested that the changes of SCD-1 and LYSC levels may be related to the lipid in tear, and may be participate in the occurrence and development of DES symptoms.

内蒙古自治区自然科学基金面上项目（2019MS08142）