目的：研究七氟醚对大鼠离体心脏全心缺血-再灌注心律失常及电生理的影响。方法：30个心脏灌注模型制备成功的SD大鼠，以K-H液对其进行15min的灌注，然后将SD大鼠均等分为3个组别，即对照组、再灌注组、七氟醚组。对照组大鼠再利用K-H液对其进行105min的灌注；再灌注组大鼠先利用K-H液对其进行15min的灌注，再利用浓度为20ml/kg的Thomas液（4℃）使大鼠心脏停止跳动1h，在4℃Thomas液对心脏进行低温保护的前提下，以浓度为10ml/kg的Thomas液（4℃）对大鼠心脏进行30min的灌注，结束1h后，再利用K-H液对大鼠心脏进行30min的灌注；七氟醚组：在使用的K-H液中添加1.0MAC强度的七氟醚，其余操作与再灌注组一致。对比3组大鼠在再灌注期间发生心律失常的情况及心脏复跳时间，同时记录4个灌注时间点的HR（心率）、左心室前壁内外膜层心肌单相动作单位，4个灌注时间点分别为T0（3组均衡灌注15min）、T1（继续灌注15min）、T2（再灌注15min/继续灌注105min）、T3（再灌注15min/继续灌注120min），记录MAPD50（单相动作电位复极50％）、MAPD90（单相动作电位复极90％）、TDR（跨室壁复极离散度）。结果：T2及T3时间点时，再灌注组大鼠的HR水平较T0、T1时显著降低（P＜0.05）；T2及T3时间点时，七氟醚组大鼠的HR水平较再灌注组明显上升（P＜0.05）；七氟醚组的心律失常持续时间以及心脏复跳时间较再灌注组明显更短（P＜0.05）；与再灌注组相比，七氟醚组在T2、T3时MAPD90缩短，TDR减少（P＜0.05）。结论：七氟醚可降低大鼠离体心脏全心缺血-再灌注的MAPD90及TDR水平，并促进此过程中心脏的复跳以及缩短心律失常的持续时间，降低心律失常的发生风险。

To study the effect of sevoflurane on arrhythmia and electrophysiology of isolated rat heart during whole-heart ischemia reperfusion.Method:Thirty adult male healthy SD(Sprague Dawley) rats were selected as the research object.Thirty Langendor heart perfusion models were selected and perfused with K-H solution for 15min.Then SD rats were equally divided into three groups,namely control group,reperfusion group and sevoflurane group.Rats in the control group were perfused with K-H solution for 105min.In reperfusion group,rats were perfused with K-H solution for 15min,then stopped beating for 1h with Thomas solution with concentration of 20ml/kg(4℃),and perfused with Thomas solution with concentration of 10ml/kg (4℃) for 30min under the premise of low temperature protection of the heart with Thomas solution at 4℃.After 1h,rats were perfused with K-H solution for 30min.Sevoflurane group:sevoflurane with 1.0MAC strength was added to the used K-H solution,and the rest operations were the same as those of reperfusion group.Arrhythmia and cardiac rebound time during reperfusion were compared among the three groups of rats.HR (heart rate) and monophasic action units of myocardium in inner and outer membranous layer of left ventricular anterior wall were recorded at four perfusion time points,namely T0 (balanced perfusion for 15min in the three groups),T1 (continuous perfusion for 15min in the reperfusion group),T2 (continuous perfusion for 15min in the sevoflurane group and 105min in the control group),T3 (continuous perfusion for 30min in the sevoflurane group and continuous perfusion for 120min in the reperfusion group).Result:At T2 and T3,HR level in reperfusion group was significantly lower than that at T0 and T1 (P<0.05).At T2 and T3 time points,HR level in sevoflurane group was significantly higher than that in reperfusion group (P<0.05).Arrhythmia duration and heart rebound time in sevoflurane group were significantly shorter than those in reperfusion group (P<0.05).Conclusion:Sevoflurane can reduce the levels of MAPD90 and TDR in isolated rat heart during whole-heart ischemia-reperfusion and promote heart rebound,shorten the duration of arrhythmia and reduce the risk of arrhythmia.