目的：探讨舌苔脱落细胞凋亡相关蛋白Bcl-2、Bax、Fas表达水平与大肠癌脾胃虚弱证的关联性。方法：用免疫组化方法,检测舌苔脱落细胞凋亡相关蛋白Bcl-2、Bax、Fas的阳性表达率。结果：三组间Bcl-2、Bax蛋白阳性表达率有统计学意义,P＜0.05；Fas蛋白阳性表达率无统计学意义,P＞0.05。结论：Bcl-2蛋白高表达,抑制细胞凋亡,Bax蛋白低表达,使细胞凋亡能力减弱,共同作用使舌苔细胞的生存期延长,细胞凋亡速率减慢,这可能是导致肿瘤细胞增殖与凋亡失衡,引发细胞恶性增殖而发为癌症的主要原因。

OBJECTS: To investigate the relationship between the expression levels of apoptosis-related proteins Bcl-2, Bax and Fas in the exfoliated cells of tongue coating and the syndrome of spleen and stomach weakness in colorectal cancer. METHODS: Immunohistochemical method was used to detect the expression of Bcl-2 and the positive expression rate of Bax and Fas in exfoliated cells. RESULTS: After statistical analysis, the positive expression rates of Bcl-2 and Bax protein in the three groups were statistically significant (P<0.05). The positive expression rate of Fas protein was not statistically significant (P>0.05). CONCLUSION: Bcl-2 protein has high expression, inhibits apoptosis, and Bax protein has low expression, which weakens the ability of apoptosis. The combined effect is to prolong the survival of tongue coating cells and slow down the apoptosis rate, which may lead to tumor cell proliferation. Unbalanced with apoptosis, causing malignant proliferation of cells and causing cancer.

内蒙古自治区高等学校科学研究重点项目(NJZZ18097)；内蒙古自治区草原英才创新团队“中医治未病”项目(项目编号：CYYCTD2018)；内蒙古自治区自然科学基金( 2019MS08162)；国家中医药管理局中医药传承与创新“百千万人才工程”第四批全国中医(临床、基础)优秀人才研修项目(No.J20184832009)