目的：探讨Sirt1对动脉粥样硬化中人冠状动脉内皮细胞炎症反应的影响。方法：选取人冠状动脉内皮细胞，分别用不同浓度的ox-LDL诱导并筛选出最适浓度建立体外动脉粥样硬化细胞模型；将细胞分为正常对照组、空质粒对照组、空质粒+ox-LDL组、Sirt1过表达+ox-LDL组、Sirt1干扰+ox-LDL组；用荧光显微镜观察质粒转染情况；qPCR和Western Blot验证Sirt1、ICAM-1的表达情况；用ELISA法检测炎症因子IL-1、hs-CRP的表达水平。结果：100 mg/L ox-LDL诱导体外动脉粥样硬化细胞模型效果最佳；与正常对照组和空质粒对照组相比，空质粒+ox-LDL组ICAM-1、IL-1、hs-CRP表达升高；与空质粒+ox-LDL组相比，Sirt1过表达+ox-LDL组ICAM-1、IL-1、hs-CRP表达降低，而Sirt1干扰+ox-LDL组则升高。结论：Sirt1能够抑制人冠状动脉内皮细胞中由ox-LDL诱发的炎症反应。

Objective:Investigate the effect of Sirt1 on inflammatory response of human coronary artery endothelial cells in atherosclerosis.Method(s):The human coronary artery endothelial cells were selected to induce and screen out the optimal concentration with different concentrations of ox-LDL to establish an in vitro atherosclerotic cell model. The cells were divided into normal control group, empty plasmid control group, empty plasmid+ox-LDL group, Sirt1 overexpression+ox-LDL group and Sirt1 interference+ox-LDL group by different treatment methods. The plasmid transfection was observed by fluorescence microscope, the expression of Sirt1、ICAM-1 was verified by qPCR and Western Blot, and the expression level of inflammatory factor IL-1、hs-CRP was detected by ELISA method.Result(s): 100 mg/L ox-LDL can effectively induce atherosclerotic cell model in vitro; Compared with normal control group and empty plasmid control group, the ICAM-1、IL-1、hs-CRP expression of empty plasmid+ox-LDL group was increased; Compared with empty plasmid+ox-LDL group, the expression of Sirt1 overexpression group was decreased.Conclusion(s):Sirt1 can inhibit ox-LDL induced inflammatory response in human coronary artery endothelial cells.

内蒙古自治区卫生计生科研计划项目