［］目的 研究蒙药格勒图呼和治疗胃癌的作用机制。方法 采用正交试验设计法筛选蒙药格勒图呼和的最佳提取工艺；采用体外细胞实验考察格勒图呼和提取物对胃癌细胞增殖迁移、凋亡以及克隆形成的影响；采用网络药理学分析和western blot验证相结合的方法研究格勒图呼和提取物治疗胃癌的信号通路。结果 格勒图呼和的最佳提取工艺为加入8倍量70%的乙醇提取1小时,连续提取2次；格勒图呼和提取物能够抑制胃癌细胞增殖、迁移和克隆形成,促进胃癌细胞凋亡,且具有剂量依赖性；格勒图呼和提取物能够抑制胃癌细胞中JNK、p38、ERK和p65的磷酸化,降低CXCL8的分泌。结论 格勒图呼和提取物通过MAPK/NF-κB/CXCL8信号通路发挥抗肿瘤作用,作为治疗胃癌的民族药值得深入研究。

［］ Objective To study the mechanism of Mongolian medicine Geletuhuhe in the treatment of gastric cancer. Methods Orthogonal experimental design was used to optimize the best extraction technology of Mongolian medicine Geletuhuhe. The effects of Geletuhuhe extract on the proliferation, migration, apoptosis and clone formation of gastric cancer cells were investigated by cell experiment in vitro. Network pharmacological analysis and Western blot verification were used to study the signaling pathway of Geletuhuhe extract in the treatment of gastric cancer. Results The best alcohol extraction condition was that the herbal medicine should be extracted for 2 times and 1 hours every time after adding 8 times of 70% ethanol; Geletuhuhe extract could inhibit the proliferation, migration and clone formation of gastric cancer cells, and promoted the apoptosis of gastric cancer cells in a dose-dependent manner. Geletuhuhe extract could inhibit the phosphorylation of JNK, p38, ERK and p65 in gastric cancer cells, and reduce the secretion of CXCL8. Conclusion Geletuhuhe extract can play an anti-tumor role through MAPK/NF-κB/CXCL8 signaling pathway, and it is worthy of further study as an ethnic medicine for the treatment of gastric cancer.