目的：本研究通过对HIF-1α、ET-1与iNOS进行蛋白表达检测，分析HIF-1α、ET-1与iNOS三者在子痫前期患者胎盘组织中的表达规律，对目前尚未完全明确的子痫前期发病机制做完善和补充。方法：通过临床收集正常妊娠产妇（对照组）、子痫前期及重度子痫前期患者胎盘组织各30例，采用 SP 免疫组化和Western-blotting方法检测各组胎盘组织中HIF-1α、ET-1及iNOS的蛋白表达部位及表达量。结果：1、免疫组化：HIF-1α及ET-1在疾病较重的胎盘组织中阳性率及阳性表达强度更高，比较三组差异有统计学意义；iNOS在各组胎盘组织中阳性率均较低，比较三组差异无统计学意义。2、Western-blotting：①重度子痫前期组中HIF-1α的表达水平明显高于正常妊娠组，差异具有统计学意义（P＜0.05）；且重度子痫前期组中HIF-1α的表达水平明显高于子痫前期组，差异具有统计学意义（P＜0.05）。②与正常妊娠组相比较，ET-1的表达水平在患病组明显增强，且重度子痫前期组ET-1 的表达水平明显高于子痫前期组，差异具有统计学意义（P＜0.05）。③iNOS在重度子痫前期组中为高表达水平，与正常妊娠组相比较表达量有明显差异，差异具有统计学意义（P＜0.05），与子痫前期组相比较表达量也有明显差异，差异具有统计学意义（P＜0.05）。结论：随着子痫前期严重程度的增加，HIF-1α、ET-1及iNOS的表达呈增强趋势，在重度子痫前期组中的表达明显高于正常妊娠组及子痫前期组，分析三者呈正相关，共同参与了子痫前期的发病机制，且与该病严重程度密切相关。

Objective: In this study, the protein expression of HIF-1α, ET-1 and iNOS was detected, and the expression rules of HIF-1α, ET-1 and iNOS in placental tissue of patients with preeclampsia were analyzed. To improve and supplement the pathogenesis of preeclampsia that is not yet fully understood. Methods: The placental tissues of normal pregnant women (set as the normal control group), preeclampsia and severe preeclampsia patients (n=30) were collected with the protein expression location and levels of HIF-1α, ET-1 and iNOS in the placental tissues of each group detected by SP immunohistochemistry and Western-blotting. Results: 1.The results of SP immunohistochemistry staining showed that the positive staining rate and relative expression intensity of HIF-1α and ET-1 were higher in the placental tissues from the patients with severe disease with the statistically significant difference found among three groups. The positive staining rate of iNOS in the placental tissues was low in all groups with no significant difference found among three groups. 2. The results of Western-blotting showed that the expression level of HIF-1α in the severe preeclampsia group was significantly higher than that in the normal control group (P<0.05). In addition, the expression level of HIF-1α in the severe preeclampsia group was significantly higher than that in preeclampsia group (P<0.05). Compared with the normal control group, the expression levels of ET-1 in the preeclampsia and severe preeclampsia groups were significantly increased, meanwhile, the expression level of ET-1 in the severe preeclampsia group was significantly higher than that in the preeclampsia group (P<0.05). We also found that the expression level of iNOS was significantly up-regulated in the severe preeclampsia group in comparison with the normal control group (P<0.05). Moreover, significant differences in the iNOS expression level were found between the preeclampsia group and severe preeclampsia groups (P<0.05). Conclusions: The expression of HIF-1α, ET-1 and iNOS increased with the severity of preeclampsia. The expression in the severe preeclampsia group was significantly higher than that in the normal pregnancy group and the preeclampsia group, and the three were positively correlated, participating in the pathogenesis of preeclampsia and closely related to the severity of the disease.

内蒙古自治区自然科学基金面上项目