目的:利用网络药理学和分子对接技术对艾迪注射液治疗鼻咽癌的作用机制进行初步探讨。方法：从TCMSP 及TCM Database@Taiwan 数据库中收集和分析艾迪注射液的有效化学成分，在TCMSP 和Swiss Target Prediction 网站预测作用靶点，在GeneCards、DisGeNET、OMIM 和TTD 数据库中检索鼻咽癌的相关靶点。绘制艾迪注射液治疗鼻咽癌的活性成分—靶点网络图，运用拓扑学方法筛选其核心化学成分。将二者交集靶点在STRING 数据库和Metascape 数据库分别进行蛋白质互作分析、GO 富集及KEGG 富集分析。最后运用Auto Dock软件对核心活性成分和关键靶点进行分子对接验证。结果：经筛选整理得到艾迪注射液有63 种活性成分和对应的852 个靶点，鼻咽癌有1989 个潜在靶点，其中二者共同靶点有308 个。应用GO 数据库功能富集分析得到310 个GO条目,应用KEGG 数据库富集筛选得到198 条有关通路，其中包含PI3K/AKT 信号通路、HIF-1α信号通路、TNF 信号通路等已报道与鼻咽癌相关的通路。分子对接结果显示，艾迪注射液的核心化学成分（quercetin、kaempferol、isorhamnetin、7-O-methylisomucronulatol 及Jaranol）对SRC、STAT3、MAPK1、PIK3R1 及RELA 蛋白具有良好的亲和力。结论：对艾迪注射液治疗鼻咽癌的作用机制进行了初步探索，并对其药效物质基础进行了预测，为进一步明确艾迪注射液治疗鼻咽癌的有效成分和作用机制提供依据。

Objective:To explore the mechanism of Aidi Injection in the treatment of nasopharyngeal carcinoma by network pharmacology and molecular docking technology. Methods:Collect and analyze the effective chemical components of Aidi Injection from TCMSP and TCM database@Taiwan database, and predict the action Target on TCMSP and Swiss Target Prediction website. The related targets of nasopharyngeal carcinoma were searched in GeneCards, DisGeNET, OMIM and TTD databases. The active component-target network diagram of Aidi Injection in the treatment of nasopharyngeal carcinoma was drawn, and its core chemical components were screened by topology method. Protein interaction analysis, GO enrichment analysis and KEGG enrichment analysis were performed in STRING database and Metascape database. Finally, AutoDock software was used for molecular docking verification of core active ingredients and key targets.Results:The results showed that There were 63 active components and 852 corresponding targets in Aidi Injection, and there were 1989 potential targets in nasopharyngeal carcinoma. There were 308 common targets. 310 GO items were obtained by functional enrichment analysis of GO database, and 198 related pathways were obtained by enrichment and screening of KEGG database, including PI3K/AKT signaling pathway, HIF-1α signaling pathway, TNF signaling pathway and other pathways that have been reported related to nasopharyngeal carcinoma. Molecular docking results showed that the core chemical components of Aidi Injection (quercetin, kaempferol, isorhamnetin, 7-o-methylismucronulatol and jaranol) had good affinity for SRC, STAT3, MAPK1, PIK3R1 and RELA proteins.Conclusion:The mechanism of Aidi Injection in the treatment of nasopharyngeal carcinoma was preliminarily explored, and the pharmacodynamic material basis was predicted. It is expected that this result can provide help for further confirming the effective components and mechanism of Aidi Injection in the treatment of nasopharyngeal carcinoma.

内蒙古自治区科技创新引导项目（KCBJ2018021）；内蒙古自然科学基金项目（2020MS08132）；2020年第十批草原英才团队项目；内蒙古医科大学青年基金（YKD2018QNCX012）