目的 探讨鹤草酚对前列腺癌细胞增殖、细胞周期和凋亡的影响。 方法 通过细胞计数、菌落形成试验、细胞周期试验、细胞凋亡试验等检测细胞增殖、细胞周期和细胞凋亡的变化。通过蛋白印迹法（western blotting）检测细胞凋亡相关蛋白的表达。通过小鼠异种移植瘤模型研究了鹤草酚在体内对肿瘤发生的影响。 结果 鹤草酚抑制了DU145细胞和PC-3细胞的增殖，降低了细胞存活率。与对照组相比，经鹤草酚处理后，S期DU145细胞和PC-3细胞的比例增加，而G2/M期的细胞比例降低。鹤草酚通过激活caspase 3相关的线粒体依赖性凋亡通路，促进DU145细胞和PC-3细胞凋亡。体内实验证实，鹤草酚能减缓前列腺癌细胞的形成，延长移植瘤小鼠的生存时间。 结论 鹤草酚是一种潜在抗癌药物，它通过激活凋亡通路，来抑制前列腺癌细胞的增殖和细胞周期进展。

Objective： To investigate the effects of agrimophol on the proliferation, cell cycle and apoptosis of prostate cancer cells. Methods： The changes in cell proliferation, cell cycle and apoptosis were detected by cell counting, colony formation assay, cell cycle assay and cell apoptosis assay. The expression of apoptosis-related proteins was detected by western blotting. The effects of agrimophol on tumorigenesis in vivo were investigated by a mouse xenograft tumor model. Results： Agrimophol inhibited the proliferation of DU145 cells and PC-3 cells and decreased the rates of cell survival. Compared to the control group, the percentage of DU145 cells and PC-3 cells in S phase increased and the percentage of cells in G2/M phase decreased after treated with agrimophol. Agrimophol promoted the apoptosis of DU145 cells and PC-3 cells through the activation of caspase 3-related mitochondria-dependent apoptotic pathway. In vivo experiments confirmed that agrimophol slowed down the formation of prostate cancer cells and prolonged the survival of tumor-bearing mice. Conclusions： Agrimophol is a potential anticancer drug that inhibits the proliferation of prostate cancer cells and the progression of cell cycle by activating the apoptotic pathway.

浙江省卫生计生委面上项目（2019KY65）