目的：观察蒙药博仁通拉嘎对慢性肾功能不全大鼠保护作用。方法：用50mg/kg/d腺嘌呤连续12w灌胃Wister雄性大鼠同时用阳性对照药尿毒清和三组不同剂量的蒙药博仁通拉嘎干预。观察用药前后大鼠体态、毛发颜色、活动状况等一般状态和肾功，甲状腺、肾上腺、性腺激素水平，肾组织炎性介质、α-SMA和NF-KB P65蛋白表达水平以及观察大鼠肾脏组织病理变化。结果：用蒙药博仁通拉嘎干预后慢性肾功能不全大鼠低剂量组的一般状态恢复最明显；与模型组比较蒙药低剂量组CRE水平降低（p<0.05）、蒙药低剂量组24h尿蛋白有降低趋势与正常无显著差异（p>0.05），蒙药低、中、高剂量组Urea的水平有降低趋势，其中蒙药低剂量组效果较好。与模型组比较蒙药低剂量组采食量增多趋势，而P和TCHO也有降低趋势。与模型组比较蒙药低、中、高剂量组LH和COR的水平显著下降（p<0.01）、T、T3和T4的水平显著升高（p<0.01），其中蒙药低剂量作用比较好。与模型组比较蒙药低、中、高剂量组IL-6，TNF-α和HIF-1的水平显著降低（p<0.01）、蒙药低剂量组TGF-β1和Ang-Ⅱ的水平显著降低（p<0.01），蒙药中高剂量组TGF-β1的水平降低（p<0.05）。蒙药低剂量组NF-KB P65和α-SMA的蛋白表达有下降趋势比尿毒清较好。结论：蒙药博仁通拉嘎对腺嘌呤诱导的慢性肾功能不全大鼠有显著的保护和防治作用，其中剂量为0.31/kg/d的保护作用最明显。作用机制可能与调节体内激素水平，改善肾功能，减少肾脏炎症反应、降低“三高”、抑制肾小管上皮细胞间质转化和ECM沉积有关。

abstract: Object: To observe the protective effect of Mongolian drug Borentonglaga on rats with chronic renal insufficiency. Methods: Wister male rats were gavaged with 50 mg/kg/d adenine in 12w at the same time lavage the Niaoduqing and three different doses of Mongolian borentonglaga. The general status, hair color, activity status and renal work, thyroid, adrenal, and gonadal hormones, the expression levels of inflammatory media: α-SMA;NF-KB(P65), and the histopathological changes were observed. Results: Compared with the model group, the CRE level was reduced in the low dose group of Mongolian drugs (p <0.05), the 24h urinary protein in the low dose group was not significantly different from normal (p> 0.05), and the level of Urea was decreased in the low, medium and high dose groups, and the effect was better in the low dose group.Compared with the model group, the trend of increased food intake in the low-dose Mongolian drug group,and P and TCHO also have a tendency to decrease.Compared with the model group, the levels of LH and COR decreased significantly in low, medium and high doses (p <0.01), T, T3 and T4 increased significantly (p <0.01), and the effect of low dose was better. Compared with the model group, the levels of IL-6 and HIF-1, TGF-β1 and Ang-Ⅱdecreased significantly in the low-dose group (p <0.01), and TGF-β1 decreased in the high-dose group (p <0.05). Moreover, the protein expression of NF-KB P65 and α-SMA decreased better than Niaoduqing group. Conclusion: The Mongolian drug Borentonglaga showed significant protective and preventive effects in rats with adenine-induced chronic renal insufficiency, with the most pronounced protection at a dose of 0.31/kg/d. The mechanism of action may be related to the regulation of hormone levels in vivo, improving renal function, reducing renal inflammatory response, reducing the renel "three high", inhibiting the stromal transformation of renal tubular epithelial cells and ECM deposition.

内蒙古自治区自然科学(编号2019MS08015)