摘要：目的 观察潞党参对慢性脑缺血（CCI）小鼠脑血流量（CBF）及认知功能的影响。方法 微弹簧圈狭窄C57 BL/6 J小鼠双侧颈总动脉，激光散斑血流成像系统观察小鼠术前及术后多个时间点（2 h，2 d，4 d，7 d，14 d，30 d）前额叶局部 CBF ，应用旷场实验、新物体识别实验和 Morris 水迷宫实验等三个行为学方法评价认知功能。结果 术后小鼠前额叶 CBF 骤降，以术后2 h 最明显，至术后30 d 仍低于正常水平，同时小鼠出现认知功能障碍。旷场实验观察到各组之间自主行为无差异，相较于假手术组，模型组新物体识别指数降低（P<0.05），逃避潜伏期显著增长（P<0.001），穿过平台次数减少（P<0.05），第Ⅲ象限的时间及路程百分比显著降低（P<0.01）。相较于模型组，潞党参高剂量组新物体识别指数显著升高（P<0.01），潞党参中、高剂量组穿越平台的次数增加（P<0.05，P<0.01），逃避潜伏期降低（P<0.01，P<0.001）；潞党参低、中、高剂量组在第Ⅲ象限停留时间及路程百分比明显提高（P<0.05，P<0.01，P <0.001）。结论 双侧颈总动脉狭窄法可造成小鼠出现慢性 CBF 下降进而导致小鼠出现持续性认知功能障碍；潞党参能够增加 CCI 小鼠局部 CBF ，改善认知功能，且具有量效关系。

Abstract: Objective To observe the effect of Ludangshen on cerebral blood flow (CBF) and cognitive function in mice with chronic cerebral ischemia (CCI). Methods The bilateral common carotid arteries of C57BL/6J mice were stenosed with microcoils. The local CBF of prefrontal lobe was observed by laser speckle blood flow imaging system before and after operation (2h, 2d, 4d, 7d, 14d, 30d). Three behavioral methods such as open field test, new object recognition test and Morris water maze test were used to evaluate cognitive function. Results After surgery, the prefrontal lobe CBF decreased significantly, which was most obvious 2 h after surgery, and was still lower than the normal level 30 d after surgery, while the mice showed cognitive dysfunction. Compared with sham operation group, new object recognition index decreased (P<0.05), escape latency increased significantly (P<0.001), the number of crossing platform decreased (P<0.05), and the percentage of time and distance in quadrant Ⅲ decreased significantly (P<0.01). Compared with model group, the new object recognition index of High dose group was significantly increased (P<0.01), the number of crossing platform was increased (P<0.05, P<0.01), and the escape latency was decreased (P<0.01, P<0.001). The percentage of stay time and distance in quadrant ⅲ in low, medium and high dose groups of Ludangshen was significantly increased (P<0.05, P<0.01, P<0.001). Conclusion Bilateral common carotid artery stenosis can cause chronic decrease of CBF in mice and lead to persistent cognitive impairment in mice. Ludangshen can increase local CBF and improve cognitive function in CCI mice in a dose-dependent manner.

山西省重点研发计划（国际科技合作）重大区域创新合作项目（201803D421006）；山西中医药大学科技创新能力培育计划（2021PY-QN-10）