目的：建立同时测定血浆中米氮平、舍曲林、帕罗西汀、度洛西汀、阿米替林、去甲阿米替林、艾司西酞普兰及劳拉西泮8种抗抑郁类药物浓度的高效液相色谱-串联质谱法（HPLC-MS/MS）。方法：内标为度洛西汀-d7，使用甲醇将血浆样品进行蛋白沉淀后分析。Waters Cortecs C18(2.1mm×50.0mm，2.7μm)色谱柱， 0.1%甲酸水溶液-0.1%甲酸甲醇溶液梯度洗脱，流速0.5mL/min，电喷雾阳离子源。通过实验对这一方法的专属性、精密度、回收率等进行考察。结果：米氮平、帕罗西汀、艾司西酞普兰在1~200ng?mL-1；舍曲林、去甲阿米替林、度洛西汀、阿米替林及劳拉西泮在2.5~500 ng?mL-1范围内均表现良好的线性关系，回收率在94.70%~101.31%之间，精密度（RSD）小于15%。结论：该方法在临床同时测定服药患者血液中抗抑郁药物的浓度时相较其他方法具有快速、灵敏、准确的特点，且具有专属性强，重复性好，稳定性高的优点。

Objective: To establish a HPLC-MS/MS method for simultaneous determination of mirtazapine, sertraline, paroxetine, duloxetine, amitriptyline, nortriptyline, escitalopram and lorazepam in human plasma. Methods: The plasma sample was precipitated with methanol after the addition of internal standard (duloxetine-d7). The chromatographic separation was performed on Waters Cortecs C18 (2.1mm×50.0mm，2.7μm) with the mobile phase of 0.1% formic acid water-0.1% formic acid methanol at a flow rate of 0.5 mL in each minutes, an electrospray ionization ion-trap source(ESI)was used as the detector and operated in the positive ion mode. The specificity, precision and recovery, of the method were investigated. Results: The liner calibration curves of mirtazapine, paroxetine, escitalopram were obtained in the concentration range of 1~200ng?mL-1; sertraline, duloxetine, amitriptyline, nortriptyline, lorazepam in the concentration range of 2.5~500ng?mL-1 respectively. The relative recovery was in the range of 94.70%~101.31%. The RSDs of inter-day and intra-day precision were less than15%. Conclusion: The method is rapid, sensitive, accurate, with high specificity, good repeatability and high stability, and it is suitable for simultaneous determination of antidepressant drug concentrations in patient blood.

内蒙古自然科学(编号:2019LH02001)